human formalin-fixed paraffinembedded (ffpe) liver tissue array  (Biomax Inc)

Journal: Clinical and Experimental Medicine

Article Title: TESC associated with poor prognosis enhances cancer stemness and migratory properties in liver cancer

doi: 10.1007/s10238-024-01469-y

Figure Lengend Snippet: Association between TESC gene expression and clinical prognosis. A High and low expression groups, established by dividing the TCGA-LIHC on the basis of median expression of CD133, CD90, and EpCAM. Thirteen intersecting differentially expressed genes were identified, with high expression of TESC and QSOX1 being noted in LCSCs from spheroid cultures. B TESC’s high expression in the TCGA-LIHC was significantly correlated with shorter overall survival (OS). C TESC expression in various cancers compared with that in adjacent normal tissues (if available), assessed using TIMER2.0. D RNA expression of the TESC gene in liver cancer and normal liver tissues from the ICGC database. E Protein expression of the TESC gene in liver cancer and normal liver tissues from the CPTAC database. F Immunohistochemistry (IHC) image of TESC in liver cancer and adjacent liver tissues of tissue microarray (Cat. LV1505a, TissueArray.Com). Scale bar, 60 μm. * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: Human liver cancer with matched cancer adjacent liver tissue array was obtained from TissueArray.Com (Cat. LV1505a, USA).

Techniques: Gene Expression, Expressing, RNA Expression, Immunohistochemistry, Microarray

Journal: PLoS ONE

Article Title: E3 Ubiquitin Ligase Synoviolin Is Involved in Liver Fibrogenesis

doi: 10.1371/journal.pone.0013590

Figure Lengend Snippet: (A) The serum levels of alanine aminotransferase (ALT) were analyzed at 0, 3, 24, 48, and 72 h after CCl 4 administration in the acute hepatic injury model mice. Data are represented as mean ± SEM (n = 4–5 mice per group). Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01. (B) Time-course expression of KLF6, synoviolin, Acta2 (α-SMA), and COL1A1 (collagen I) mRNA in livers inoculated with CCl 4 (black bar) or vehicle (white bar) quantified using real-time RT-PCR. Data are represented as mean ± SEM (n = 4–5 mice per group). The mRNA level was normalized relative to the amount of the transcript of 18S rRNA, a housekeeping gene. Unpaired Student's t -test was used for statistical analysis. *** P <0.001, ** P <0.01, * P <0.05. (C) Immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4) or vehicle (n = 5). The expression and localization of synoviolin (arrows, right panel) were analyzed using anti-synoviolin antibodies. Normal IgG antibody was used for the negative control (left panels). Scale bar = 100 µm. (D) Double-labeled fluorescent immunohistochemical analysis of liver sections of wt mice at 48 h after treatment with CCl 4 (n = 4). The nuclei were counterstained with DAPI (Vectashield). The expression and localization of synoviolin (green) and α-SMA (red)—a marker of activated HSCs, were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 200 µm. (E) Double-labeled fluorescent immunohistochemical analysis of liver sections of human healthy liver (n = 30) and cirrhotic liver (n = 40) using human tissue array sections. The expression and localization of synoviolin (green label) α-SMA (red label) were analyzed using anti-synoviolin and α-SMA antibodies. Scale bar = 100 µm.

Article Snippet: Pathologically confirmed human liver tissue arrays were obtained from U.S. Biomax (Rockville, MD).

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemical staining, Negative Control, Labeling, Marker